GMP DNA 5'-O--Thymidine 3'-CE Phosphoramidite CAS 98796-51-1

5'-O--thymidine 3'-CE phosphoramidite is a building block for the introduction of thymidine units into oligonucleotides. 5'-O--thymidine 3'-CE phosphoramidite is utilised in a broad range of applications, including the synthesis of natural and unnatural DNA and RNA strands. Phosphoramidites are the reagent class of choice for oligonucleotide synthesis as they contain readily removable orthogonal protecting groups and can be produced at reasonable scales. The diisopropylamino leaving group is readily cleaved upon exposure to an azole catalyst allowing for a highly efficient coupling reaction with a free hydroxyl group on the growing oligonucleotide and oxidation of the subsequent phosphite triester, followed by beta-elimination of the cyanoethyl group affords the stable phosphodiester linkage.

-dT Phosphoramidite belongs to the class of DNA Phosphoramidites.The key features of standard protected DNA phosphoramidite are:

• Exocyclic amine functions are protected by a benzoyl group (dA(bz) and dC(bz)) or isobutyryl group (dG(ib))
• Recommended cleavage and deprotection conditions are 8 hours at 55 °C or 24 hours at room temperature using concentrated ammonia solution for standard base-protected oligonucleotides
• The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides (a)

SPECIFICATION

5'-O--thymidine 3'-CE phosphoramidite

TECHNICAL SPECIFICATION

Product Code: C-015

Synonyms: dT-CE phosphoramidite 5'-Ditrityl-2'-deoxythymidine, 3'-[(2-cyanoethyl)-(N,Ndiisopropyl)]-phosphoramidite

CAS Number: 98796-51-1

Chemical Formula: C40H49N4O8P

Molecular Weight: 744.83

Appearance: White to pale yellow crystalline powder

Purity (HPLC): min 99%

Identity ( 31P NMR): Conforms to structure